Single Nucleotide Polymorphisms as Human Disease Markers Essay Example for Free

Single Nucleotide Polymorphisms as Human Disease Markers Essay In using single nucleotide polymorphisms (SNPs) to match subtle DNA changes with an individual’s susceptibility to a disease of interest, one can start by distinguishing the suspect DNA from a preset standard using the technique of restriction fragment length polymorphism (RFLP). In this method, copies of extracted and purified DNA are made using the polymerase chain reaction (PCR) before being cut up by preselected restriction endonucleases, and the resulting fragments subjected to electrophoresis. Once a suspect sequence in the fragments obtained is found, it can then be subjected to a sequencing technique that can pinpoint the nature of the SNP. One such method is Pyrosequencing, where a sequencing primer is prepended to the fragment under scrutiny and is mixed with a cocktail composed of a DNA polymerase, ATP sulfurylase, luciferase (the enzyme responsible for making luciferin in luminous animals glow), and apyrase, as well as luciferin and adenosine 5’-phosphosulfate (APS). Deoxyribonucleotide triphosphates (dNTPs, four in total, corresponding to the four DNA bases) are added, one by one, into the reaction mix. For each time a dNTP is added, the polymerase causes the incorporation of the dNTP into the strand at appropriate positions, if any are present, with the simultaneous release of pyrophosphate, in an amount that is equimolar to how much nucleotide was used in incorporation. The pyrophosphate reacts with the APS, catalyzed by ATP sulfurylase, to produce ATP which participates in the chemoluminescence reaction of luciferin and luciferase. The amount of visible light generated by the reaction is proportional to the amount of ATP generated, and this is recorded by a special camera and noted as a peak into what is called a Pyrogram. How much light is generated depends on how much dNTP was used up. Any unused dNTP and ATP present in the mix is degraded by the apyrase. The sequence of the fragment can then be read off from the peaks of the gradually being produced Pyrogram. Specially constructed software can then spot SNPs by comparing the Pyrogram of the fragment of interest with known Pyrograms. By using an â€Å"association study†, where people with the trait and a control group are compared with regards to the presence of â€Å"marker alleles†, or alleles that can be shown to be present only in people with the disease. SNPs are convenient for this purpose since SNPs are easily inheritable and occur usually more frequently in genes than the other possible variations. This is most useful for diseases that can be shown to be influenced by only a single gene, for association is difficult enough when done on a single gene. Accuracy in identification can be had by having the affected and the control be as closely related as possible, since differences that might be disease-related will be easier to spot. References: Ronagi, M. Elahi, E. (2002). Discovery of single nucleotide polymorphisms and mutations by Pyrosequencing. Comparative and Functional Genomics, 3(1), 51-56. Li, M., Boehnke, M., Abecasis, G. R. (2006). Efficient study designs for test of genetic association using sibship data and unrelated cases and controls, The American Journal of Human Genetics, 78, 778–792.

The Destruction of Family in 1984 :: essays research papers

The family unit of Oceania in George Orwell’s book, 1984, plays an important part to society. These families are broken rather than households of affection and comfort. Oceania’s government, called the Party, controls the families in every aspect. With these non-existent families, there is a cycle of breaking down of family and a stronger Party as times passes until a there is force strong enough to end it. These families that lead to corruption in society should be avoided in order to prevent a totalitarian government from rising. In order to keep their power, the government purposefully breaks up families in Oceania. The pieces of these hollow and artificial families are the building blocks of the vast and manipulative Party. Families need to be non-existent so that the people cannot unite or feel loved. However, the Party also needs to have total control over the children. In Oceania, it is normal to turn other people in when you have any suspicions that the person does not have genuine love for the Party. Even family members give each other up. The government-controlled families create a cycle of breaking down of family and a stronger Party. The future families that will produce more probes disguised as children. With more children taught to continue the legacy of the Party, there is increased authority of the government. As government gains more power, the people lose will over their own lives. This should not be the way of life for people, where you can be erased from history at any given time. There is no doubt that there are many families that are just as and maybe more inexpressive and false than the families in Oceania. However, the greater masses of the people don’t believe in a strict society of absolute control. If enough families were to border upon the Parsons family, then the domino line would be in effect. Through his book, Orwell attempts to warn us that the destruction of families is dangerous. If allowed to continue, the government will have too much control over a mindless society that could be turne d any which way the ruling class chooses. Ultimately, dysfunctional families create a selfish and unsuspecting society that is ruled by a government of great dominance over the people. The Destruction of Family in 1984 :: essays research papers The family unit of Oceania in George Orwell’s book, 1984, plays an important part to society. These families are broken rather than households of affection and comfort. Oceania’s government, called the Party, controls the families in every aspect. With these non-existent families, there is a cycle of breaking down of family and a stronger Party as times passes until a there is force strong enough to end it. These families that lead to corruption in society should be avoided in order to prevent a totalitarian government from rising. In order to keep their power, the government purposefully breaks up families in Oceania. The pieces of these hollow and artificial families are the building blocks of the vast and manipulative Party. Families need to be non-existent so that the people cannot unite or feel loved. However, the Party also needs to have total control over the children. In Oceania, it is normal to turn other people in when you have any suspicions that the person does not have genuine love for the Party. Even family members give each other up. The government-controlled families create a cycle of breaking down of family and a stronger Party. The future families that will produce more probes disguised as children. With more children taught to continue the legacy of the Party, there is increased authority of the government. As government gains more power, the people lose will over their own lives. This should not be the way of life for people, where you can be erased from history at any given time. There is no doubt that there are many families that are just as and maybe more inexpressive and false than the families in Oceania. However, the greater masses of the people don’t believe in a strict society of absolute control. If enough families were to border upon the Parsons family, then the domino line would be in effect. Through his book, Orwell attempts to warn us that the destruction of families is dangerous. If allowed to continue, the government will have too much control over a mindless society that could be turne d any which way the ruling class chooses. Ultimately, dysfunctional families create a selfish and unsuspecting society that is ruled by a government of great dominance over the people.

Merchant Banking

Merchant Banking Merchant banking may be defined as, â€Å"an institution which covers a wide range of activities such as management of customer services, portfolio management, credit syndication, acceptance credit, counselling, insurance, etc. † Merchant Banks are popularly known as â€Å"issuing and accepting houses†. They offer a package of financial services. Unlike in the past, their activities are now primarily non-fund based. One of the basic requirements of merchant banks is highly professional staff with skills and worldwide contacts.The basic function of merchant banks is marketing corporate and other securities, that is guaranteeing sales and distribution of securities. All the aspects- origination, underwriting and distribution of the sale of industrial securities are handled by them. They are experts and good judges of the type, timing and terms of issues and make them acceptable to investors under prevailing preferences and market conditions, and at the sa me time afford the borrowing company, flexibility and freedom that it needs to meet possible future contingencies.They guarantee the success of issues by underwriting them. They also provide all the services related to receiving applications, allotment, collecting money, sending share certificates and so on. The merchant banker normally does not assume all the risk himself while underwriting the issue. Merchant banks offer services also to investors. The range of activities offered by merchant banks is much wider than sponsoring public issues of industrial securities. They offer project finance, syndication of credit, corporate advisory services, mutual fund investments, investment management etc.Services of Merchant Banks †¢ Project Counselling: Project counselling includes preparation of project reports, deciding upon the financing pattern to finance the cost of the project and appraising the project report with the financial institutions or banks. It also includes filling up of application forms with relevant information for obtaining funds from financial institutions and obtaining government approval. †¢ Issue Management: Management of issue involves marketing of corporate securities viz. equity shares, preference shares and debentures or bonds by offering them to public.Merchant banks act as an intermediary whose main job is to transfer capital from those who own it to those who need it. After taking action as per SEBI guidelines, the merchant banker arranges a meeting with company representatives and advertising agents to finalise arrangements relating to date of opening and closing of issue, registration of prospectus, launching publicity campaign and fixing date of board meeting to approve and sign prospectus and pass the necessary resolutions. Pricing of issues is done by the companies in consultant with the merchant bankers. †¢ Underwriting of Public Issue:Underwriting is a guarantee given by the underwriter that in the event of under subscription, the amount underwritten would be subscribed by him. Banks/Merchant banking subsidiaries cannot underwrite more than 15% of any issue. †¢ Managers, Consultants or Advisers to the Issue: The managers to the issue assist in the drafting of prospectus, application forms and completion of formalities under the Companies Act, appointment of Registrar for dealing with share applications and transfer and listing of shares of the company on the stock exchange. Companies can appoint one or more agencies as managers to the issue. Portfolio Management: Portfolio refers to investment in different kinds of securities such as shares, debentures or bonds issued by different companies and government securities. Portfolio management refers to maintaining proper combinations of securities in a manner that they give maximum return with minimum risk. †¢ Advisory Service Relating to Mergers and Takeovers: A merger is a combination of two companies into a single company where one s urvives and other loses its corporate existence. A takeover is the purchase by one company acquiring controlling interest in the share capital of another existing company.Merchant bankers are the middlemen in setting negotiation between the two companies. †¢ Off Shore Finance: The merchant bankers help their clients in the following areas involving foreign currency. (a) Long term foreign currency loans (b) Joint Ventures abroad (c) Financing exports and imports (d) Foreign collaboration arrangements †¢ Non-resident Investment: The services of merchant banker includes investment advisory services to NRI in terms of identification of investment opportunities, selection of securities, investment management, and operational services like purchase and sale of securities. †¢ Loan Syndication:Loan syndication refers to assistance rendered by merchant bankers to get mainly term loans for projects. Such loans may be obtained from a single development finance institution or a s yndicate or consortium. Merchant bankers help corporate clients to raise syndicated loans from banks or financial institutions. †¢ Corporate Counselling: Corporate counselling covers the entire field of merchant banking activities viz. project counselling, capital restructuring, public issue management, loan syndication, working capital, fixed deposit, lease financing acceptance credit, etc. bibliography a manual on merchant banking by JC Verma Merchant Banking Merchant Banking Merchant banking may be defined as, â€Å"an institution which covers a wide range of activities such as management of customer services, portfolio management, credit syndication, acceptance credit, counselling, insurance, etc. † Merchant Banks are popularly known as â€Å"issuing and accepting houses†. They offer a package of financial services. Unlike in the past, their activities are now primarily non-fund based. One of the basic requirements of merchant banks is highly professional staff with skills and worldwide contacts.The basic function of merchant banks is marketing corporate and other securities, that is guaranteeing sales and distribution of securities. All the aspects- origination, underwriting and distribution of the sale of industrial securities are handled by them. They are experts and good judges of the type, timing and terms of issues and make them acceptable to investors under prevailing preferences and market conditions, and at the sa me time afford the borrowing company, flexibility and freedom that it needs to meet possible future contingencies.They guarantee the success of issues by underwriting them. They also provide all the services related to receiving applications, allotment, collecting money, sending share certificates and so on. The merchant banker normally does not assume all the risk himself while underwriting the issue. Merchant banks offer services also to investors. The range of activities offered by merchant banks is much wider than sponsoring public issues of industrial securities. They offer project finance, syndication of credit, corporate advisory services, mutual fund investments, investment management etc.Services of Merchant Banks †¢ Project Counselling: Project counselling includes preparation of project reports, deciding upon the financing pattern to finance the cost of the project and appraising the project report with the financial institutions or banks. It also includes filling up of application forms with relevant information for obtaining funds from financial institutions and obtaining government approval. †¢ Issue Management: Management of issue involves marketing of corporate securities viz. equity shares, preference shares and debentures or bonds by offering them to public.Merchant banks act as an intermediary whose main job is to transfer capital from those who own it to those who need it. After taking action as per SEBI guidelines, the merchant banker arranges a meeting with company representatives and advertising agents to finalise arrangements relating to date of opening and closing of issue, registration of prospectus, launching publicity campaign and fixing date of board meeting to approve and sign prospectus and pass the necessary resolutions. Pricing of issues is done by the companies in consultant with the merchant bankers. †¢ Underwriting of Public Issue:Underwriting is a guarantee given by the underwriter that in the event of under subscription, the amount underwritten would be subscribed by him. Banks/Merchant banking subsidiaries cannot underwrite more than 15% of any issue. †¢ Managers, Consultants or Advisers to the Issue: The managers to the issue assist in the drafting of prospectus, application forms and completion of formalities under the Companies Act, appointment of Registrar for dealing with share applications and transfer and listing of shares of the company on the stock exchange. Companies can appoint one or more agencies as managers to the issue. Portfolio Management: Portfolio refers to investment in different kinds of securities such as shares, debentures or bonds issued by different companies and government securities. Portfolio management refers to maintaining proper combinations of securities in a manner that they give maximum return with minimum risk. †¢ Advisory Service Relating to Mergers and Takeovers: A merger is a combination of two companies into a single company where one s urvives and other loses its corporate existence. A takeover is the purchase by one company acquiring controlling interest in the share capital of another existing company.Merchant bankers are the middlemen in setting negotiation between the two companies. †¢ Off Shore Finance: The merchant bankers help their clients in the following areas involving foreign currency. (a) Long term foreign currency loans (b) Joint Ventures abroad (c) Financing exports and imports (d) Foreign collaboration arrangements †¢ Non-resident Investment: The services of merchant banker includes investment advisory services to NRI in terms of identification of investment opportunities, selection of securities, investment management, and operational services like purchase and sale of securities. †¢ Loan Syndication:Loan syndication refers to assistance rendered by merchant bankers to get mainly term loans for projects. Such loans may be obtained from a single development finance institution or a s yndicate or consortium. Merchant bankers help corporate clients to raise syndicated loans from banks or financial institutions. †¢ Corporate Counselling: Corporate counselling covers the entire field of merchant banking activities viz. project counselling, capital restructuring, public issue management, loan syndication, working capital, fixed deposit, lease financing acceptance credit, etc. bibliography a manual on merchant banking by JC Verma

A Book Analysis - Tom Standages A History of the World in 6 Glasses Free Essay Example, 1500 words

The international trading of wine from Greece and other proximate areas that have vineyards and wineries initiated the beginnings of long-voyages and trading, as well as the initiation of scientific revolutions such as the invention of distillation, most of which continued to flourish well into the ages of explorations, colonization, and unfortunately even during the numerous wars and revolutions that resulted from these ventures. The distillation process gradually helped the wine to evolve into other products such as distilled spirits like brandy, as well as the invention of other alcoholic products sourced from sugar- or carbohydrate-rich foods such as sugarcane and corn, mostly grown in the New World and other parts of Africa. However, the rise in the alcohol market allowed the proliferation of slavery in many regions of the world and exploitation of vast tracts of land and its people, the taxation of commodities previously deemed as waste, and in the New World, particularly in w hat is now known as the United States the uprising of the colonizers against England. Due to such circumstances, wine and spirits became symbols of oppression as these helped to shape the modern world by helping the inhabitants of the Old World to establish themselves as rulers of the New World. Similar to the circumstances surrounding the creation of distilled spirits, a relatively newer drink invented in the U. S.We will write a custom essay sample on A Book Analysis - Tom Standages A History of the World in 6 Glasses or any topic specifically for you Only $17.96 $11.86/page